Spectrophotometry for Cerebrospinal Fluid Pigment Analysis
نویسندگان
چکیده
منابع مشابه
Spectrophotometry for cerebrospinal fluid pigment analysis: a review
The use of spectrophotometry for the analysis of the cerebrospinal fluid (CSF) is reviewed. The clinically relevant CSF pigments — oxyhemoglobin and bilirubin — are introduced and discussed with regard to clinical differential diagnosis and potentially confounding variables (the four “T”s or: traumatic tap, timing, total protein and total bilirubin). The practical laboratory aspects of spectrop...
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We describe a two-stage method for measuring thromboplastin in cerebrospinal fluid, based on the amidolytic determination of the generation rate of Factor Xa. We investigated the effect of variations in the duration of incubation, and in pH, temperature, and concentrations of calcium, buffer, and prothrombin complex (source of Factors VII and X). Optimal assay conditions are specified. The dete...
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Spectrophotometric examinations were performed on cerebrospinal and subdural fluids in subacute (five patients) and chronic (20 patients) subdural haematomas, with special reference to the diagnostic aid of CSF spectrophotometry. Spectrophotometric xanthochromia of haemorrhagic origin was found in all CSFs examined, while definite visible xanthochromia was observed in only 28% and the CSF was j...
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This method for direct determination of Zn in cerebrospinal fluid (CSF) involves flame atomic absorption spectrophotometry with a pulse nebulizer technique. Standard solutions of Zn in 150 mmol/L NaCl were used. We could account for 88% of added standard with the method in individual samples from 10 patients and in pooled CSF. The method is acceptably precise, CVs in pooled CSF ranging from 4 t...
متن کاملVisual inspection versus spectrophotometry in detecting bilirubin in cerebrospinal fluid.
OBJECTIVES To compare the diagnostic accuracy of visual inspection and spectrophotometry for identifying the presence of bilirubin in the cerebrospinal fluid (CSF). METHODS Clinicians and students assessed CSF specimens with seven degrees of extinction between 0.00 and 0.09 at 450-460 nm as "yellow," "doubtful," or "colourless" after random presentation under standard conditions. The assessme...
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ژورنال
عنوان ژورنال: Neurocritical Care
سال: 2006
ISSN: 1541-6933
DOI: 10.1385/ncc:4:2:153